Friday Lecture 11/7

Exam 3 Review

• Clathrin pinching
  • PI(4.5)P_2
• COPI and Clathrin usually use ARF proteins
• COPII usually uses Sar1 proteins
• GEF promotes vesicle assembly, and GAP promotes vesicle disassembly.
  • Assembly is faster than disassembly.
• t-SNARE and v-SNAREs make the trans-SNARE complex.
• NSF protein unbinds the trans-SNARE complex
• Anterograde transport goes forward via COPII, and retrograde transport goes back via COPI.
• The KDEL sequence and KDXX sequence are both used for transport
• Know the CGN, TGN, and that there is a cisterna
• Review complex oligosaccharides.
• O-linked glycosylation gets its own enzyme.